Expression of Survivin and Caspase-3 in cholangiocarcinoma and the correlative research

Objective To investigate expression of Survivin and Caspase-3 in cholangiocarcinoma and their relationship.Methods Immunohistochemical assay(streptavidin-biotin peroxidase method) was used to detect the expression of Survivin and Caspase-3 in 42 cholangiocarcinoma tissues,20 adjacent noncancerous bile duct lesions and 11 benign bile duct lesions.The chi-square test and association analysis were used to evaluate the difference of significance.The SPSS12.0 software package was used to conduct statistical analysis.The P value 0.05 was thought as statistical significant.Results ①Expression of Survivin was significantly higher in cholangiocarcinoma(61.9%) than that in adjacent noncancerous(5%) and benign bile duct lesions(0%)(P0.01).Expression of Caspase-3 was significantly lower in cholangiocarcinoma(45.2%) than that in adjacent noncancerous(85.0%) and benign bile duct lesions(90.9%)(P0.01).②Expression of Survivin in cholangiocarcinoma tissues increased with the reduction of the differentiation level of tumor(rs=0.404,P 0.01),whereas expression of Caspase-3 in cholangiocarcinoma tissues decreased with the reduction of the dif-ferentiation level of tumor(rs=-0.389,P0.05).③The negative correlativity between the expression of Survivin and Caspase-3 could be found with statistical significance(rs=-0.765,P0.01).Conclusion High expression of Survivin and low expression of Caspase-3 depress the apoptosis of cholangiocarcinoma,which maybe related to the growth of cholangiocarcinoma.The negative correlativity of the expression between Survivin and Caspase-3 suggests that Survivin inhibit the apoptosis induced by Caspase-3,which maybe related to the occurrence and development of cholangiocarcinoma.