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Real-time fluorescence quantitative polymerase chain reaction for detection of neisseria gonorrhoeae infection

Lihong Zhao, Hong Yu, Lili Li, Ming Zhao

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Abstract

Objective To detect Neisseria gonorrhoeae DNA in genitourinary specimens by real-time fluorescence quantitative Polymerase Chain Reaction (FQ-PCR), and to provide a clinical basis for the diagnosis and treatment of gonorrhoea. Methods The study consisted 173 specimens, which were detected for DNA with Neisseria gonorrhoeae real-time FQ-PCR. The specimens were cultured as well. Results Of the 173 clinical specimens, real-time FQ-PCR showed that 47 were positive(27.2%);while 41 cases(23.7%) were positive by the culture method. Compared to the culture method, the specificity, sensitivity, and agreement of FQ-PCR was 95.5%, 100%, and 96.5%, respectively. Conclusion FQ-PCR is a simple, rapid, sensitive and specific method to detect Neisseria gonorrhoeae.

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What this paper is about

Objective To detect Neisseria gonorrhoeae DNA in genitourinary specimens by real-time fluorescence quantitative Polymerase Chain Reaction (FQ-PCR), and to provide a clinical basis for the diagnosis and treatment of gonorrhoea. Methods The study consisted 173 specimens, which were detected for DNA with Neisseria gonorrhoeae real-time FQ-PCR. The specimens were cultured as well. Results Of the 173 clinical specimens, real-time FQ-PCR showed that 47 were positive(27.2%);while 41 cases(23.7%) were positive by the culture method. Compared to the culture method, the specificity, sensitivity, and agreement of FQ-PCR was 95.5%, 100%, and 96.5%, respectively. Conclusion FQ-PCR is a simple, rapid, sensitive and specific method to detect Neisseria gonorrhoeae.

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Available abstract

Objective To detect Neisseria gonorrhoeae DNA in genitourinary specimens by real-time fluorescence quantitative Polymerase Chain Reaction (FQ-PCR), and to provide a clinical basis for the diagnosis and treatment of gonorrhoea. Methods The study consisted 173 specimens, which were detected for DNA with Neisseria gonorrhoeae real-time FQ-PCR. The specimens were cultured as well. Results Of the 173 clinical specimens, real-time FQ-PCR showed that 47 were positive(27.2%);while 41 cases(23.7%) were positive by the culture method. Compared to the culture method, the specificity, sensitivity, and agreement of FQ-PCR was 95.5%, 100%, and 96.5%, respectively. Conclusion FQ-PCR is a simple, rapid, sensitive and specific method to detect Neisseria gonorrhoeae.

Key concepts: Neisseria gonorrhoeae, Polymerase chain reaction, Real-time polymerase chain reaction, Microbiology, Medicine, Neisseria, Gonococcal infection, Neisseriaceae

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