Studies on Anti-Browning During Tissue Culture of Musella lasiocarpa

Callus necrosis during in vitro propagation is a large obstacles in breeding and large scale production of Musella lasiocarpa. To solve this problem,the effects of sterilizing methods of explants,the ratio of plant growth regulators and 4 kinds of browning inhibitors on callus browning were studied with the callus induced from its immature male flowers as the explants. The results indicated that the contamination rate of unsterilized explants was 0%,and its browning rate and browning index were all significantly lower than that of the explants after disinfection; 6-BA played a major role in callus induction of M. lasiocarpa. It conduced to callus induction and significantly reduced browning index when the concentration of 6-BA was 2 mg·L- 1or 3 mg·L- 1. Adding VC on the surface and VC mixture of citric acid failed to suppress browning. However,Ginger and ferulic acid gave satisfactory result to reduced browning,48. 5 mg·L- 1ferulic acid could significantly reduce the callus browning and increased the rate of differentiation to17. 9%,15% higher than that of the control.