EXPRESSION OF bcl-2 GENE IN ACUTE MYELOID LEUKEMIA CELLS AND THE CLINICALSIGNIFICANCE

Objective:To study the clinical significance on the expression of bcl 2 gene in acute myeloid leukemia cells.Methods:Expression of bcl 2 gene was detected in mononuclear cell of bone marrow from 57 patients with in situ hybridization with streptavidin gold (ISH SAG).Results:There were different expressions in 57 patients.The scope is 0～98 %,the positive rate was 46.2 % in primary group ; 40.7 % in complete remission ( CR ) group ; 100 % in relapsed or refractory group . The expression was significantly higher in the relapsed or refractory group than that in the primarygroup and CR group ( P 0.01 ) . No relationship was observed between the expression of bcl 2 gene and FAB type ( P 0.05 ) . It was found that the expression of bcl 2 gene was related to the clinical remission.CR rate was 42.1 % in bcl 2 positive cases and 76.2 % in bcl 2 negative group ( P 0.05).Conclusion:The overexpression of bcl 2 gene were relevant to the poor prognosis.The expression of bcl 2 gene was detected to explore its predictive values,which might be helpful to guide the using of drugs in clinic.