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Expression of Glycoprotein B(gB)of Infectious Laryngotracheitis Virus Strain Wanggang in Insect Cells Infected with a Recombinant Baculovirus

Zhang Shao

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Abstract

The gB gene of infectious laryngotracheitis virus(ILTV)strain WG was subcloned into the Baculovirus transfer vector pVL1393 and a recombinant vector rpVLgB was generated. Co_transfection of Sf9 insect cells with rpVLgB and Baculovirus linear DNA was performed (BAC_N_Blue DNA).After three times purification,a recombinant baculovirus designated as rpVL_ILTVgB was obtained. Expression of ILTV gB in rpVL_ILTVgB infected Sf9 cells was detected by direct immunofluorescence and Dot_ELISA.The expressed glycoprotein B will be used as subunit vaccine or diagnostic antigen.

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What this paper is about

The gB gene of infectious laryngotracheitis virus(ILTV)strain WG was subcloned into the Baculovirus transfer vector pVL1393 and a recombinant vector rpVLgB was generated. Co_transfection of Sf9 insect cells with rpVLgB and Baculovirus linear DNA was performed (BAC_N_Blue DNA).After three times purification,a recombinant baculovirus designated as rpVL_ILTVgB was obtained. Expression of ILTV gB in rpVL_ILTVgB infected Sf9 cells was detected by direct immunofluorescence and Dot_ELISA.The expressed glycoprotein B will be used as subunit vaccine or diagnostic antigen.

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Available abstract

The gB gene of infectious laryngotracheitis virus(ILTV)strain WG was subcloned into the Baculovirus transfer vector pVL1393 and a recombinant vector rpVLgB was generated. Co_transfection of Sf9 insect cells with rpVLgB and Baculovirus linear DNA was performed (BAC_N_Blue DNA).After three times purification,a recombinant baculovirus designated as rpVL_ILTVgB was obtained. Expression of ILTV gB in rpVL_ILTVgB infected Sf9 cells was detected by direct immunofluorescence and Dot_ELISA.The expressed glycoprotein B will be used as subunit vaccine or diagnostic antigen.

Key concepts: Sf9, Recombinant DNA, Virology, Biology, Baculoviridae, Molecular biology, Glycoprotein, Recombinant virus

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Expression of Glycoprotein B(gB)of Infectious Laryngotracheitis Virus Strain Wanggang in Insect Cells Infected with a Recombinant Baculovirus — Research Paper | ScholarLens