2010•Unpublished venueRequires access
Baculovirus surface display of cap protein of PCV2.
Xing FuShan, Xingang Xu, Dewen Tong, Zhisheng Wang, Yanming Zhang
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Abstract
In this study,the ORF2 gene of Porcine circovirus typeⅡ(PCV2) deleting N-terminal nuclear localization region was cloned into vector pBacSC to form the recombinant plasmid.The recombinant plasmid was transformed into E.coli DH10Bac.The recombinant baculovirus DNA was screened and identified by blue-white plaque assay and antibotic resisitant selection.Recombinant baculovirus were obtained from Sf9 insect cells in which recombinant baculovirus DNA was transfected by the liposome.Cap protein was expressed and displayed on the viral surface,as revealed by western blot and immunogold electron microscopy.The successful construction of recombinant baculovirus BacSC-Cap would be useful for production of gene vaccination in the future.