Effects of Resveratrol on Function of Peritoneal Macrophages in Severe Acute Pancreatitis

Objective:To investigate the role of peritoneal macrophages(PM) in the pathogenesis of severe acute pancreatitis(SAP) in rats,and observe the effect of Resveratrol(Res) on function of PM in SAP.Methods:Thirty-six rats were divided into three groups: sham operation group(SO)、SAP group and Res group.rats were sacrificed 4 h and 12 h after the flasking.PM were extracted,and incubated for 24 h in a humidified 5% carbon dioxide incubator.The expressions of tumor necrosis factor alpha(TNF-α) mRNA and interleukin-1β(IL-1β) mRNA in PM were measured by semi-quantitative RT-PCR.The levels of TNF-α and IL-1β in culture medium and serum were evaluated.The histological changes in pancreas were examined.Results:To contrast with SAP group,scores of pancreatic are lightened in Res group,The expressions of TNF-α mRNA and IL-1β mRNA in PM were significantly higher in SAP group than SO group at each time point;The concentrations of TNF-α and IL-1β in culture medium and serum were significantly increased in SAP group when compared with those in SO group(P0.001).To contrast with SAP group,The expressions of TNF-α mRNA、IL-1β mRNA in PM and the concentrations of TNF-α and IL-1β in culture medium and serum were significantly descended in Res group(P0.001),but all of index were higher than those in SO group(P0.05).Conclusions:PM could play an important role in SAP pathogenesis.Res can relieve the injury of pancreas in rats with SAP,the mechanism of which may be related to the inhibitory effect of Res on the function of PM by inhibition of expression of TNF-a and IL-1β mRNA.