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2008•Lishizhen Medicine and Materia Medica ResearchRequires access

The TLC Identification for Four Medicinal Materials and the Determination of Icariin in Tianshenyizhi Capsule

Zhizhong Guan

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Abstract

Objective To establish the quality standard for Tianshenyizhi Capsule.Methods Herba Epimedii,Radix Angelicae Sinensis,Rhizoma Acori Tatarinowii,Radix Polygoni Multiflori cum Succo Glycines Sotae in Tianshenyizhi Capsule were identified by TLC,and the content of icariin was determined by HPLC.Results The identification method was highly exclusive.Icariin showed a good linear relationship in the range of 0.322~1.61μg,and r=0.999 98.The average recovery was 100.7%,RSD= 1.43%(n= 9).Conclusion The method is accurate and simple with good reproducibility,and it can control the quality of this preparation effectively.

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What this paper is about

Objective To establish the quality standard for Tianshenyizhi Capsule.Methods Herba Epimedii,Radix Angelicae Sinensis,Rhizoma Acori Tatarinowii,Radix Polygoni Multiflori cum Succo Glycines Sotae in Tianshenyizhi Capsule were identified by TLC,and the content of icariin was determined by HPLC.Results The identification method was highly exclusive.Icariin showed a good linear relationship in the range of 0.322~1.61μg,and r=0.999 98.The average recovery was 100.7%,RSD= 1.43%(n= 9).Conclusion The method is accurate and simple with good reproducibility,and it can control the quality of this preparation effectively.

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Available abstract

Objective To establish the quality standard for Tianshenyizhi Capsule.Methods Herba Epimedii,Radix Angelicae Sinensis,Rhizoma Acori Tatarinowii,Radix Polygoni Multiflori cum Succo Glycines Sotae in Tianshenyizhi Capsule were identified by TLC,and the content of icariin was determined by HPLC.Results The identification method was highly exclusive.Icariin showed a good linear relationship in the range of 0.322~1.61μg,and r=0.999 98.The average recovery was 100.7%,RSD= 1.43%(n= 9).Conclusion The method is accurate and simple with good reproducibility,and it can control the quality of this preparation effectively.

Key concepts: Icariin, Radix (gastropod), Quality standard, Chromatography, Capsule, High-performance liquid chromatography, Traditional medicine, Chemistry

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