Extraction of total flavonoids from Naodesheng by orthogonal design

Objective:To confirm the optimum extraction of the total flavonoids s from Naodesheng.Methods:The orthogonal test was used to optimize the extracting condition.Total flavonoids determined by colorimetr and puerarin and hydrox-ysafflor yellow A determined by HPLC were taken as the evaluating markers.The effects of different extraction methods on the extraction efficiency of the flavonoids were investigated.Results:The optimum extraction conditions were refluxed with 10,8,6 times amount of 70% alcohol and for 3 times(2 h,1 h,1 h).Conclusion:The contents of the flavonoids obtained were high by the optimum extraction conditions.The technique is simple and adapts for production.