HPLC determination of puerarin in plasma and saliva samples

To establish determination of puerarin in plasma and saliva samples by HPLC.Chromatography column was ODS C18 Column(250 mm×4.6 mm,Yilite company in Dalian);Mobile phase was acetonitrile-water(containing 0.8% glacial acetic acid,15∶ 85,V/V);the flow-rate was 0.8 mL/min;temperature at 30 ℃ and detection wave length at 250 nm.In plasma and saliva,standard curve of Puerarin ranged with 5.0~-350.0 μg/mL and 0.1~5.0 μg/mL respectively,the LOQ was 5.0 μg/mL and 0.1 μg/mL,the average recovery was 97.5%~103.0% and 86.4%~108.0%,the RSD of intra-day was 3.3%~6.4% and 2.7%~3.9%,the RSD of inter-day was 1.6%~3.6and 3.5%~4.6%.The high performance liquid chromatographic(HPLC) method could be used simultaneously for determination of puerarin in plasma and saliva samples,which is sensitive and simple for small-biological samples analysis.It was suggested that the methodology study may be used in clinical pharmacokinetics of puerarin.