RAPD analysis of genetic diversity of non-heading Chinese cabbage(Brassica campestris ssp.chinensis Makino) germplasm

In order to lay a theoretical foundation for digging genetic potential of non-heading Chinese cabbage,and to provide molecular evidence for origin and evolution of non-heading Chinese cabbage,the genetic diversity of 64 accessions of non-heading Chinese cabbage(Brassica campestris ssp.chinensis Makino)germplasm was analysed by RAPD(random amplified polymorphic DNA).147 alleles were detected by 23 RAPD primers,71 of which(48.30%)were polymorphic among different accessions.Averagely,6.39 alleles and 3.09 polymorphic alleles per primer were amplified.The Nei′s gene diversity and Shannon′s information index of B.compestris var.communis were 0.126 9 and 0.194 6,respectively,which were the highest among different types,and they were 0.123 7 and 0.188 8,respectively,which were also the highest among different ecological regions.The Nei′s gene diversity and Shannon information index of B.compestris ssp.chinensis in China were the higher than in other countries.The genetic differentiation coefficient of non-heading Chinese cabbage was 59.05%,which showed that the most of variances were distributed mainly among populations.The gene flow was 0.499 5,which showed that the genes flowed less among populations.