2010Journal of Southwest China Normal UniversityRequires access

ISSR Analysis of Genetic Diversity of 27 Flowering Chinese Cabbage

Yin Cai-xia

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Abstract

Genetic diversity of 27 flowering Chinese cabbage (Brassica campestris L.ssp.chinensis var.utilis Tssen.et Lee) cultivars were analyzed with inter-simple sequence repeat (ISSR) molecular markers.A total of 103 loci were detected using 12 ISSR primers,of which 58 were polymorphic,the percentage of polymorphic loci (p) being 56.31%.Shannon's information index (I) and Nei's gene diversity (H) were 0.229 and 0.144,respectively,observed number of alleles (Na) and effective number of alleles (Ne) were 1.563 and 1.225,respectively,and the genetic distance ranged from 0.029 to 0.344,indicating that the genetic diversity of flowering Chinese cabbage was relatively low.UPGMA cluster analysis clustered the 27 cultivars into six groups.

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Genetic diversity of 27 flowering Chinese cabbage (Brassica campestris L.ssp.chinensis var.utilis Tssen.et Lee) cultivars were analyzed with inter-simple sequence repeat (ISSR) molecular markers.A total of 103 loci were detected using 12 ISSR primers,of which 58 were polymorphic,the percentage of polymorphic loci (p) being 56.31%.Shannon's information index (I) and Nei's gene diversity (H) were 0.229 and 0.144,respectively,observed number of alleles (Na) and effective number of alleles (Ne) were 1.563 and 1.225,respectively,and the genetic distance ranged from 0.029 to 0.344,indicating that the genetic diversity of flowering Chinese cabbage was relatively low.UPGMA cluster analysis clustered the 27 cultivars into six groups.

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Available abstract

Genetic diversity of 27 flowering Chinese cabbage (Brassica campestris L.ssp.chinensis var.utilis Tssen.et Lee) cultivars were analyzed with inter-simple sequence repeat (ISSR) molecular markers.A total of 103 loci were detected using 12 ISSR primers,of which 58 were polymorphic,the percentage of polymorphic loci (p) being 56.31%.Shannon's information index (I) and Nei's gene diversity (H) were 0.229 and 0.144,respectively,observed number of alleles (Na) and effective number of alleles (Ne) were 1.563 and 1.225,respectively,and the genetic distance ranged from 0.029 to 0.344,indicating that the genetic diversity of flowering Chinese cabbage was relatively low.UPGMA cluster analysis clustered the 27 cultivars into six groups.

Key concepts: UPGMA, Genetic diversity, Biology, Brassica, Allele, Cultivar, Microsatellite, Botany

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