2006•Xibei Nong-Lin Keji Daxue xuebao. Ziran kexue banRequires access
Isolation and culture of rat marrow-derived mesenchymal stem cells in vitro
Xia Liu
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Abstract
To investigate the isolation and culture of mesenchymal stem cells (MSCs) in vitro from rat bone marrow,a steady culture system of MSCs for future study was established. The mononuclear cells derived from rat marrow were obtained from rat bone marrow by density gradient centrifugation and selected by cell attachment. The morphology of MSCs was observed by microscope. The cell cycle and surface antigens were identified by flow cytometry. The growth curve of cells was analysed through MTT experiment. The results showed that MSCs were fibroblast-like cells. They were positive for CD29 and CD44,but were negative for CD45 and HLA-DR. About 95% of MSCs were in G0/G1 phase and MSCs had some primordial cells characteristics. Morphology of MSCs was singula and their growing was stable. In addition, proliferation of MSCs was high.