Influence and mechanism of Jianpizhidong Decoction on autonomic activities in mouse model of Tourette's syndrome

Objective To determine the effect of the therapy of purging liver on Tourette's syndrome and reveal the mechanism.Method All mice(n=90) were randomly divided into the blank group,model group,tiapride suspension group,Jianpizhidong Decoction group,Liujunzi Decoction and Xieqing Pills group.Except the blank group,other 5 groups were injected intraperitoneally IDPN for establishing the model,and at the same time the blank group and model group were given normal saline solution intragastrically and other groups were given relevant medicinal.The autonomic activities and changes of plasma neurotransmitter content were compared in all groups.Result One day after modeling,the autonomic activities increased in the model group compared with the blank group(P0.01),decreased in the Jianpizhidong Decoction group and Xieqing Pills group compared with the model group and tiapride suspension group(P0.05,P0.01),and decreased in the Jianpizhidong Decoction group compared with the Liujunzi Decoction(P0.01).Twenty-one days after modeling,the autonomic activities increased in the model group compared with the blank group(P0.01),and decreased in the Jianpizhidong Decoction group and Xieqing Pills group compared with the model group,tiapride suspension group and Liujunzi Decoction(P0.05,P0.01).The content of plasma dopamine(DA),DOPAC,HVA and noradrenalin(NE) increased in the model group compared with the blank group(P0.05),and decreased in the Jianpizhidong Decoction group compared with the model group(P0.05).The content of HVA decreased in the Jianpizhidong Decoction group compared with the tiapride suspension group(P0.05).The content of DA and HVA decreased in the Jianpizhidong Decoction group compared with the Liujunzi Decoction(P0.05).Conclusion Jianpizhidong Decoction and Xieqing Pills can reduce the excitability of central nerve in the mice with Tourette's syndrome and Xieqing Pills plays a major role,but the therapeutic effect was not supported in the detection of neurotransmitter,which may indicate that the action has other target.