Ultrastructure and Distribution of Phenol in Phalaenopsis Browning Leaf Explants

In the browning Phalaenopsis explants,cell walls were distorted.The cell membranes as well as the cell walls were twist distorts even breakon.And there were abundant of black pellet substance gather in the degraded cell wall.The chloroplasts were shrinked to deep color structure.The results showed that there a lots of phenol in vascular tissues when the browning explant cell treated with Pauly reagent and 10% NaNO2.The integrity of cellular membrane structure of browning explant was destroyed during explant browning.The increasing of polyphenol content in explant aggravated browning.