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Experimental studies on antiproliferation activities and early apoptosis inducement of polysaccharides from Spirulina platensis

Hong Yu

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Abstract

In vitro experiments the antiproliferation activities of polysaccharides from Spirulina platensis(PSP) were evaluated on Hela cells and HepG2 cells by MTT assay,the results showed that PSP(2.5~40 mg/L,48~72 h) exhibited dose and time inhibition effects on the cell growth.The early apoptosis of Hela cells was detected by flow cytometry analysis with a combination of Annexin V-FITC and PI double staining,the apoptosis rates induced by PSP(5,10,20,40 mg/L) were 30.3%,32.6%,34.8% and 38.8% respectively,which were higher than those of the normal control group(P0.05).Meanwhile,the apoptosis rate treated by PSP(40 mg/L) also increased in time-dependent manner.These results suggested that one of the anti-tumor mechanisms of PSP may be related to inhibition on cell proliferation and induction of notable apoptosis.

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What this paper is about

In vitro experiments the antiproliferation activities of polysaccharides from Spirulina platensis(PSP) were evaluated on Hela cells and HepG2 cells by MTT assay,the results showed that PSP(2.5~40 mg/L,48~72 h) exhibited dose and time inhibition effects on the cell growth.The early apoptosis of Hela cells was detected by flow cytometry analysis with a combination of Annexin V-FITC and PI double staining,the apoptosis rates induced by PSP(5,10,20,40 mg/L) were 30.3%,32.6%,34.8% and 38.8% respectively,which were higher than those of the normal control group(P0.05).Meanwhile,the apoptosis rate treated by PSP(40 mg/L) also increased in time-dependent manner.These results suggested that one of the anti-tumor mechanisms of PSP may be related to inhibition on cell proliferation and induction of notable apoptosis.

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Available abstract

In vitro experiments the antiproliferation activities of polysaccharides from Spirulina platensis(PSP) were evaluated on Hela cells and HepG2 cells by MTT assay,the results showed that PSP(2.5~40 mg/L,48~72 h) exhibited dose and time inhibition effects on the cell growth.The early apoptosis of Hela cells was detected by flow cytometry analysis with a combination of Annexin V-FITC and PI double staining,the apoptosis rates induced by PSP(5,10,20,40 mg/L) were 30.3%,32.6%,34.8% and 38.8% respectively,which were higher than those of the normal control group(P0.05).Meanwhile,the apoptosis rate treated by PSP(40 mg/L) also increased in time-dependent manner.These results suggested that one of the anti-tumor mechanisms of PSP may be related to inhibition on cell proliferation and induction of notable apoptosis.

Key concepts: Spirulina (dietary supplement), Apoptosis, Annexin, HeLa, Flow cytometry, Polysaccharide, MTT assay, Pi

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Experimental studies on antiproliferation activities and early apoptosis inducement of polysaccharides from Spirulina platensis — Research Paper | ScholarLens