Microsatellite Separation and the Polymorphism Analysis on Carassius auratus

Objective: This study used the microsatellite primers of carp to isolate Carassius auratus L's new microsatellite markers,then analyzed the polymorphism of the two microsatellite loci.Methods: 6 pairs of microsatellite primers were used to amplify the genomic DNA in Carassius auratus L using the PCR technique,The PCR products were analyzed by 8% non-denatured polyacrylamide gelatin electrophoresis,and stained with silver,then we identified the sequences of 2 pairs of primers's PCR products which contain microsatellite DNA fragments.Results:After the sequences aligning and BLAST analysis,the results indicated that we isolated 2 new microsatellite sequences of Carassius auratus L,which contains the repeat units of TA and AC.Then we designed two primers according to the new microsatellite sequences and further amplified by PCR on C.auratus auratus to investigate polymorphism of the 2 microsatellite markers.The results show that the heterozygosis of the two microsatellite loci are 0.611 and 0.644,the polymorphism content are 0.536 and 0.572.Conclusion:which indicated that these two microsatellite loci have high polymorphism.The results indicated that the microsatellite markers of Carassius auratus of great importance in heredity multiplicity,heredity chain-like atlas construction,the molecular mark assistance breeding and the identify of idioplasm resources.