ISOLATION AND CHARACTERIZATION OF POLYMORPHIC MICROSATELLITE MARKERS OF ANOPHELES LESTERI (DIPTERA:CULICIDAE)

Microsatellite DNA sequences of Anopheles lesteri were isolated,and the polymorphic microsatellite loci were characterized. Digested genomic fragments were hybrizied with biotinylated oligonucleotide probes. The target fragments moleculars were captured with Vectrex Avidin D and enriched by centrifuged with ultra-4-column ultra-filtrate. Then these fragments containing microsatellite repeats were amplified,cloned and sequenced. The suitable microsatellite loci were chosen and established with the PCR amplification assay. The polymorphism screening was used by PAGE gel electrophoresis with wild individuals of An. lesteri. A total of 58 microsatellite DNA sequences were obtained in this study (GenBank Acession numbers: EF620299-EF620356). The percentage of perfect microsatellite DNA sequence was 70.7% (41/58),with imperfect sequence as 12.1% (7/58) and compound sequence as 15.7% (9/58). Only one was non-typical microsatellite sequence. 11 of 14 microsatellite loci were found.