Effects of Ginsenoside CK on Gastric Cancer SGC-7901 Cell Line and Endogenous VEGF Secreted by Tumor Cells

Objective To investigate the effects of Ginsenoside CK on the cell proliferation、cell cycle and endogenous VEGF of gastric cancer SGC-7901 cell line.Methods SGC-7901 cell line in cultural medium in vitro were given 50,25,12.5,6.25,3.125,1.5625 μg/ml Ginsenosde CK.The inhibitory rate of cell line was measured by MTT assay;cell apoptotic rate and cell phase were detected by flow Cytometry(FCM);the VEGF contents were detected by ELISA assay.Results It was found that Ginsenoside CK had significant inhibitory effect on the proliferation of SGC-7901 cell line,and this effect was dose-and-time-dependent;FCM assay indicated that apoptosis peak appeared evidently after the cells being interfered with Ginsenoside CK and most of cells were arrested in G0/G1 phase;The levels of VEGF in Ginsenoside CK groups were lower than that in the control group(P0.05).The level of VEGF in the groups of high concentration Ginsenoside CK were lower than that in the group of low concentration(P0.05),and the VEGF contents decrease with action time prolonging.Conclusion Ginsenoside CK dramatically inhibits proliferation of SGC-7901 cell line by inducing cell apoptosis and secretion of endogenous VEGF.Ginsenoside CK may become a potential drug in the prevention and treatment of gastric cancer.