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Induction of Apoptosis of Human Gastric Carcinoma SGC-7901 Cells with Ginsenoside Rh2

LI Jing-hua

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Abstract

Objective To study the effect of ginsenoside Rh2(G-Rh2) on human gastric carcinoma SGC-7901 cells.Methods Add G-Rh2 to SGC-7901 cells to various final concentrations.Determine the survival rate of SGC-7901 cells by MTT method,the content of apoptotic body by flow cytometry,and evaluate the activation of caspase by Western blot and determination of caspase-3/-7 activity in vitro.Results G-Rh2 showed significantly dose-dependent inhibitory effect on the growth of SGC-7901 cells.The IC50 of G-Rh2 was 9.3 μg/ml.After treatment with 7.5 μg/ml of G-Rh2 for 24 h,the apoptosis rate of SGC-7901 cells was 6.97%.Both the cleavage of poly(ADP-ribose)polymerase(PARP)and caspase-3/-7 activity appeared after treatment with 7.5 μg/ml of G-Rh2 for 20 h and increased as time goes on.Conclusion G-Rh2 induced the apoptosis of SGC-7901 cells in which caspase was involved.

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What this paper is about

Objective To study the effect of ginsenoside Rh2(G-Rh2) on human gastric carcinoma SGC-7901 cells.Methods Add G-Rh2 to SGC-7901 cells to various final concentrations.Determine the survival rate of SGC-7901 cells by MTT method,the content of apoptotic body by flow cytometry,and evaluate the activation of caspase by Western blot and determination of caspase-3/-7 activity in vitro.Results G-Rh2 showed significantly dose-dependent inhibitory effect on the growth of SGC-7901 cells.The IC50 of G-Rh2 was 9.3 μg/ml.After treatment with 7.5 μg/ml of G-Rh2 for 24 h,the apoptosis rate of SGC-7901 cells was 6.97%.Both the cleavage of poly(ADP-ribose)polymerase(PARP)and caspase-3/-7 activity appeared after treatment with 7.5 μg/ml of G-Rh2 for 20 h and increased as time goes on.Conclusion G-Rh2 induced the apoptosis of SGC-7901 cells in which caspase was involved.

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Available abstract

Objective To study the effect of ginsenoside Rh2(G-Rh2) on human gastric carcinoma SGC-7901 cells.Methods Add G-Rh2 to SGC-7901 cells to various final concentrations.Determine the survival rate of SGC-7901 cells by MTT method,the content of apoptotic body by flow cytometry,and evaluate the activation of caspase by Western blot and determination of caspase-3/-7 activity in vitro.Results G-Rh2 showed significantly dose-dependent inhibitory effect on the growth of SGC-7901 cells.The IC50 of G-Rh2 was 9.3 μg/ml.After treatment with 7.5 μg/ml of G-Rh2 for 24 h,the apoptosis rate of SGC-7901 cells was 6.97%.Both the cleavage of poly(ADP-ribose)polymerase(PARP)and caspase-3/-7 activity appeared after treatment with 7.5 μg/ml of G-Rh2 for 20 h and increased as time goes on.Conclusion G-Rh2 induced the apoptosis of SGC-7901 cells in which caspase was involved.

Key concepts: Apoptosis, Poly ADP ribose polymerase, Flow cytometry, Western blot, Molecular biology, Chemistry, IC50, In vitro

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