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2006•Guangxi Medical JournalRequires access

Rooting of tissue culture plantlets of Siratia grosvenorii

PU Rui-lin

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Abstract

Objective To probe into the favourable conditions for rooting of in vitro plantlets of Siratia grosvenorii .Methods The phytohormones of IAA , IBA , NAA 0.1 mg·L -1 were added into 1/2 MS medium respectively. The in vitro plantlets of Siratia grosvenorii without roots were planted in the 1/2 MS mediums, and grew at different levels of temperature.Results The highest induction rate 90%, was obtained in NAA treatment . The lower temperature(16℃～22℃) was beneficial for rooting, and the higher temperature(30℃) was advantageous for root growing. In the condition of temperature fluctuation from day to night, the roots grew longer and thicker.Conclusion In vitro,plantlets of Siratia grosvenorii have best rooting in the 1/2 MS medium with NAA 0.1 mg·L -1 at the day-night temperature ranging from 16℃ to 30℃.

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Objective To probe into the favourable conditions for rooting of in vitro plantlets of Siratia grosvenorii .Methods The phytohormones of IAA , IBA , NAA 0.1 mg·L -1 were added into 1/2 MS medium respectively. The in vitro plantlets of Siratia grosvenorii without roots were planted in the 1/2 MS mediums, and grew at different levels of temperature.Results The highest induction rate 90%, was obtained in NAA treatment . The lower temperature(16℃～22℃) was beneficial for rooting, and the higher temperature(30℃) was advantageous for root growing. In the condition of temperature fluctuation from day to night, the roots grew longer and thicker.Conclusion In vitro,plantlets of Siratia grosvenorii have best rooting in the 1/2 MS medium with NAA 0.1 mg·L -1 at the day-night temperature ranging from 16℃ to 30℃.

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Available abstract

Objective To probe into the favourable conditions for rooting of in vitro plantlets of Siratia grosvenorii .Methods The phytohormones of IAA , IBA , NAA 0.1 mg·L -1 were added into 1/2 MS medium respectively. The in vitro plantlets of Siratia grosvenorii without roots were planted in the 1/2 MS mediums, and grew at different levels of temperature.Results The highest induction rate 90%, was obtained in NAA treatment . The lower temperature(16℃～22℃) was beneficial for rooting, and the higher temperature(30℃) was advantageous for root growing. In the condition of temperature fluctuation from day to night, the roots grew longer and thicker.Conclusion In vitro,plantlets of Siratia grosvenorii have best rooting in the 1/2 MS medium with NAA 0.1 mg·L -1 at the day-night temperature ranging from 16℃ to 30℃.

Key concepts: Horticulture, Murashige and Skoog medium, Tissue culture, Botany, Biology, In vitro, Biochemistry

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