Recombinant human interleukin-10 inhibits expression of syndecan-4 protein in adventitial fibroblasts induced by tumor necrosis factor-α in vitro

Objective To investigate the effect of recombinant human interleukin-10(IL-10)on proliferation of adventitial fibroblasts and expression of syndecan-4 protein induced by tumor necrosis factor-α(TNF-α).Methods NIH 3T3 cells were cultured in vitro and exposed to 20 ng/mL TNF-α,100 ng/mL IL-10,200 ng/mL IL-10,100 ng/mL IL-10 with 20 ng/mL TNF-α,and 200 ng/mL IL-10 with 20 ng/mL TNF-α respectively.All groups were treated for 24 h in vitro,and were compared with the control group.The ratio of proliferation of NIH/3T3 cells was determined by non-radioactive MTS/PMS assay and the expression of syndecan-4 protein was evaluated by Western blotting using anti-syndecan-4 antibodies.Results Statistical analysis showed that,(1) Compared to the control group,TNF-α significantly stimulated the proliferation of NIH/3T3 cells at the concentration of 20 ng/mL in TNF-α group(P0.001).IL-10 alone had no effect on NIH/3T3 cells growth(P0.05),but significantly inhibited NIH/3T3 cells proliferation induced by TNF-α(P0.01).(2) Compared with the control group,the expression of syndecan-4 protein in NIH/3T3 cells was significantly enhanced by TNF-α at the concentration of 20 ng/mL(P0.05).IL-10 alone had no effect on the expression of syndecan-4 protein(P0.05),but significantly inhibited the expression of syndecan-4 induced by TNF-α(P0.001).Conclusion IL-10 could inhibit expression of syndean-4 protein in NIH/3T3 cells induced by TNF-α in vitro.