Localization and Clinical Significance of Matrix Metalloproteinase-2 mRNA Protein in the Human Laryngeal Squamous Cell Carcinoma Tissue

Objective:To study the localization and expression of matrix metalloproteinase-2(MMP-2)mRNA in human laryngeal squamous cell carcinoma tissue.Methods:The amount of expression of 50 laryngeal carcinoma patients,50 cases primary tissues lateral carcinoma,lymph node,20 cases normal tissue was analyzed by the immunohistochemical analysis.The MMP-2 protein in ISH(immunohistochemistry)was evaluated by the image analysis.In addition,the correlation between the MMP-2 mRNA expression and clinicopathological features(pathology grades,T stages,lymph node metastasis)wasevaluated.Results:The MMP-2mRNA was observed in carcinoma cell and stromal cytoplasm,Although the MMP-2mRNA expression in laryngeal carcinoma tissues was high than the lateral tissues,it was not correlated with clinicopathological features(the tumor T stage,pathology grades,lymph node metastasis).Conclusion:The MMP-2mRNA was observed in carcinoma cell and stromal cytoplasm.MMP-2mRNA expression in the primary tissues in laryngeal carcinoma was the highest among these samples,it maybe an early information of carcinoma invasion and lymph node metastasis.