Effects of rosiglitazone on the expresstion of α-SMA in proximal tubular epithelial cells in type2 diabetic rats

OBJECTIVE To study the effects of rosiglitazone on the expresstion of α-SMA in proximal tubular epithelial cells in type2 diabetic rats.METHODS Making 2 type diabetic rats mode by given high-sugar and high-fat diets,associating injection of small count streptozotocin(STZ) in abdominal cavity,to observe pathological changes in control,diabetic,and rosiglitazone-treated groups successively,and to detect the expression of α-SMA,in proximal tubular epithelial cells by immunohistochemistry,to detect α-SMA expression by flow cytometry.RESULTS 1 Symptom and sign: the diabetic rats showed the symptoms of polyuria,polydipsia,polyphagia,after injection of STZ 1 to 2 days,as the progression of DN,which became serious.At week 24,the diabetic rats'spirits dispirited,hairs dimmed,the behaviors reduced.But the weights of diabetic rats did not low in the whole progression,the rosiglitazone-treated group was similar with the diabetic group in symptom and sign.2 Biochemical examination: FBG,Ins,TG,TC,Uaer of diabetic group increased more significantly,than normal group,Ccr decreased(P0.05).Comparing with diabetic group,Ins,TC,Uaer,in rosiglitazone-treated group,decreased,but the FBG,TG,Ccr did not significantly decreased(P0.05).3 Pathological changes: Light microscopy: comparing with normal group,in diabetic group,a few of the tubules atrophy or dilataltion and the tubular epithelia cells were swelling,and light fatty degeneration in the cytoplasm.In rosiglitazone-treated group,pathological changes lightened,between the normal group and diabetic group.4 Immunohistochemical staining: comparing with normal group,in the diabetic rats the expression of α-SMA enhanced.In rosiglitazone group,expression of α-SMA was in the range of the normal group and the diabetic group.The detections of α-SMA by flow cell technic were coincidence with results of immunohistochemistry.CONCLUSION Rosiglitazone not only can relieve the insulin resistance,correct the disturbance of lipo-metabolism,decrease the Uaer,but also can relieve the expression of α-SMA in tubular epithelial cells,delay the interstitial fibrosis,to protecting kidney.