Determination of Antibiotics Residues in Raw Milk by Microbial Inhibition Method

Objective To establish a rapid method in order to detect various antibiotic residues in raw milk.Method On the principle that antibiotics could inhibit effects on sensitive bacteria,10 kinds of antibiotic residues in raw milk were rapidly detected by microbial inhibition method.Results The limits of detection of this method were as follows:ampicillin and penicillin were 0.001 μg/ml,and aureomycin,terramycin,erythromycin and tetracycline were 0.01 μg/ml,and streptomycin,chloramphenicol,gentamicin and kanamycin were 0.1 μg/ml.These results were superior to those of TTC method described in GB/T 4789.27—2003.Conclusion The results indicated that the microbial inhibition method was a low-cost,stable and sensitive method,and take only 18 to 22 hours.The limits of detection could meet national standards.10 kinds of antibiotic residues were simultaneously detected.