Urine detection of survivin in the diagnosis of bladder cancer

Objective:To investigate the potential suitability of survivin detection in urine as a novel predictive/prognostic molecular marker of bladder cancer.Methods:Survey of urine specimens from 4 groups:healthy volunteers(n=8)and patients with nonneoplastic urinary tract disease(n=9),other genitourinary cancer(n=22),new-onset or recurrent bladder cancer(n=47).Survivin levels,analyzed by ELISA detection system and confirmed by reverse transcriptase polymerase chainreaction(RT-PCR)and Western blot,in urine samples of the 4 participant groups.Results:Survivin was detected in the urine samples of the patients with bladder cancer.Survivin was not detected in the urine samples of patients without bladder cancer(P0.05).ELISA detection outcomes were confirmed by RT-PCR and Western blot.Conclusions:Highly sensitive and specific determination of urine survivin appears to provide a simple,noninvasive diagnostic test to identify patients with new or recurrent bladder cancer.