EXPRESSIONS OF COLORECTAL-CANCER-RELATED GENES:TISSUE MICROARRAYS VERSUS ORDINARY SECTIONS

Objective To evaluate the effectivity of tissue microarrays technology in immunohistichemical experiment by comparing tissue microarrays with ordinary section. Methods Paraffin block specimens of colorectal cancer of 84 patients were selected to make tissue microarrays and ordinary sections.Expressions of Bax,GST-π,LRP,P-gp and Topo Ⅱ were detected immunohistochemically. Results A significantly positive association was observed between the expressions of ordinary section and the tissue microarrays(r=0.795-0.867,P0.001).Concordance of expressions between the ordinary section and the tissue microarrays was 90.24% for Bax,93.90% for GST-π,91.46% for LRP,90.24% for P-gp,and 92.68% for Topo Ⅱ,respectively.The discordance of expressions between the ordinary section and tissue microarrays was not significant(χ2=0.125-1.500,P0.05). Conclusion Employing a normative tissue microarrays-making procedure,the array of 2 mm in diameter can replace the common section in immunohistochemical staining,which is a reliable,typical or effective high-flux tool for tissue analysis.