A Simple Making Method of Tissue Microarray and Validation Study on Immunohistochemistry

Objective To search a simple making mothd of tissue microarray and evaluate its validation. Methods A new, simple and easy method was designed to improve the technology of TMA during the experiment. Tissue microarrays with 1.6 mm in diameter tissue core were constructed from 124 cases of breast cancer. The tissue microarrays were stained for oestrogen receptor(ER),progesterone receptor(PR)and c-erBb2 using immunohistochemistry.At the same time the stains on the tissue microarrays were compared with that from the full tissue sections. Results A highly significant association was observed between the staining scores(0 ～ 7)obtained from the full tissue sections and from the tissue microarrays. Concordance for the receptor status(positive / negative)of the whole section and the tissue core were 94.35% for ER,93.55% for PR.and 91.94% for c-erBb2.The discordance between full section and tissue microarrays was studied using Two - Related - Samples tests and the result had no statistical significance(P0.05) . Conclusion Tissue microarays with 1.6 mm in diameter tissue core made by simple method can represent full tissue section on immunohistochemistry study.We believe our method of tissue microarray is a reliable and valid