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Regulating effect of 17β-estradiol on osteoprotegerin and osteoclast differentiation factor expressions in osteoblasts from newborn rats

Xiong Qian

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Abstract

Osteoblasts (OB) isolated from newborn SD rats were cultured in vitro.After treatment with different concentrations of 17β-estradiol (10~(-11)-10~(-6)mol/L),the mRNA expressions of osteoprotegerin (OPG) and osteoclast differentiation factor (ODF) in OB were measured by RT-PCR.17β-estradiol increased the expression of OPG in OB with the maximal effect at the concentration of 10~(-8) mol/L.No significant difference was observed in the expression of ODF in OB with different concentrations of 17β-estradiol.The therapeutic effect of estrogen on osteoporosis appears to be related to the enhanced OPG expression in OB at physiological concentration of estrogen.

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What this paper is about

Osteoblasts (OB) isolated from newborn SD rats were cultured in vitro.After treatment with different concentrations of 17β-estradiol (10~(-11)-10~(-6)mol/L),the mRNA expressions of osteoprotegerin (OPG) and osteoclast differentiation factor (ODF) in OB were measured by RT-PCR.17β-estradiol increased the expression of OPG in OB with the maximal effect at the concentration of 10~(-8) mol/L.No significant difference was observed in the expression of ODF in OB with different concentrations of 17β-estradiol.The therapeutic effect of estrogen on osteoporosis appears to be related to the enhanced OPG expression in OB at physiological concentration of estrogen.

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Available abstract

Osteoblasts (OB) isolated from newborn SD rats were cultured in vitro.After treatment with different concentrations of 17β-estradiol (10~(-11)-10~(-6)mol/L),the mRNA expressions of osteoprotegerin (OPG) and osteoclast differentiation factor (ODF) in OB were measured by RT-PCR.17β-estradiol increased the expression of OPG in OB with the maximal effect at the concentration of 10~(-8) mol/L.No significant difference was observed in the expression of ODF in OB with different concentrations of 17β-estradiol.The therapeutic effect of estrogen on osteoporosis appears to be related to the enhanced OPG expression in OB at physiological concentration of estrogen.

Key concepts: Osteoprotegerin, Internal medicine, Endocrinology, Osteoclast, Estrogen, Osteoporosis, Chemistry, In vitro

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Regulating effect of 17β-estradiol on osteoprotegerin and osteoclast differentiation factor expressions in osteoblasts from newborn rats — Research Paper | ScholarLens