Study of the Mechanism of Autologous Serum Cultured Human Bone Marrow Stromal Cells Differentiate into Neuron-like Cells

Objective: To investigate the probability and mechanisms of using autologous serum for the culture of human bone marrow stromai cells and induction into neuron-like cell. Methods: Bone marrow stromal cells were Separated from human bone marrow by gradient centrifugation and expanded with autologous serum in vitro. Cultured cells were evaluated by CD34, CD44, CD45 and CD105 by Flocytometry. 3~5 passage cells were induced by growth factor and N2. The morphology of induced the cells were observed under light microscope and immuocytochemistry stained for NSE. The change of expression of Nestin and NSE mRNA were detected by RT-PCR. Results: Most of the human bone marrow stromal cells were long or flat fusiform shape and expanded rapidly in vitro. CD44 and CD105 were positive in most cells while CD34 and CD45 were negative. After induced neuron-like cells were observed and 58.62%+3.68 % were NSE positive cells. Nestin mRNA expressed mostly after 3 days and decreased gradingly while NSE mRNA mostly expressed after 547 days and lasted for several days. Conclusions: Autologous sermn cultured bone marrow stromal cells can be induced into neuron-like cells and may used for the repair of neural system damage.