Cultivation of human bone marrow mesenchymal stem cells and induced differentiation into neuron-like cells

Objective To explore a way that induce human bone marrow mesenchymal stem cells to differentiate into neuron-like cells in vitro.Methods Bone marrow mesenchymal stem cells were cultivated in a medium containing serum by using density gradient centrifugation method and whole marrow method and were induced to differentiation by βmercaptoethanol.Immunohistochemistry were used to identify the type of cells.Results The number of cells was((1.73)±(0.44))×10~(7)/ml by using whole marrow method and ((7.65)±(0.52))×10~(7)/ml by using density gradient centrifugation method at the 10th day cultivation respectively.There was statistically significant difference(P(0.01)).After the induction,bone marrow mesenchymal stem cells displayed processes that formed extensive networks.Positive cells for NSE were(54.76%)±(3.65%),and for GFAP were(36.28%)±(4.27%) respectively.Conclusions The number of cells was more with density gradient centrifugation method than with whole marrow method.Bone marrow mesenchymal stem cells could eventually differentiate into neuronlike cells in vitro by β-mercaptoethanol and optimal medium.