Protection effect of Zhengyuan Prescription on myelosuppression of guinea pigs induced by cyclophosphamide

Objective To investigate the effects of Zhengyuan Prescription on the myelosuppression of guinea pigs induced by cyclophosphamide(CTX).Methods Guinea pigs were randomly divided into control group,model group,rhG-CSF group(20μg· kg-1),and three groups each containing low(2.00g·kg-1),medium(4.00g· kg-1),high(8.00g·kg-1)dose of Zhengyuan Prescription.Zhengyuan Prescription groups and rhG-CSF group were administered for 3days before modeling,while the remaining groups received physiological saline with equal volumes,and the duration of the experiment was 18days.Except for the control group,the other guinea pigs were induced by CTX(64mg·kg-1,im,for 3days)on the 4th day.Peripheral blood was observed at the 8th day after modeling.After the experiment,the organ indexes of heart,liver,spleen,lung,and kidney were measured and the changes of bone marrow smears were observed under the microscope.Results Compared with the control group,the number of peripheral white blood cell(WBC),red blood cell(RBC),lymphocyte,neutrophil and hemoglobin content of the model group decreased significantly(P0.01or P0.05).Furthermore,the results of bone marrow smear revealed that bone marrow megakaryocytes were damaged in varying degrees.Compared with the model group,WBC counts of the treatment groups were increased significantly(P0.01),especially for the group of rhG-CSF,the WBC and neutrophil counts were enhanced most remarkably(P0.01).The blood cells counts of guinea pigs in the group with medium dose of Zhengyuan Prescription were increased significantly(P0.01or P0.05),while the organ indexes of heart,liver,spleen,lung,and kidney had no significant changes(P 0.05).The results of bone marrow smear showed that the group of Zhengyuan Prescription with medium dose had a obvious therapeutic effect on the bone marrow nucleated cells and megakaryocytic cells system which was suppressed by CTX.Conclusion The recovery of peripheral blood and regeneration of bone marrow cells in myelosuppressed guinea pigs induced by CTX can be promoted by Zhengyuan Prescription which had a protective effect on the bone marrow hematopoietic system.