Change and Significance of Inflammatory Reaction and Cytokines in Induced Sputum from Patients with Chronic Obstructive Pulmonary Disease in Different Stages

Objective To explore the state and significance of airway inflammation for the patients with COPD(chronic obstructive pulmonary disease) in different stages.Methods Examining the forced expiratory volume in 1 second(FEV1.0) by pulmonary function equipment and Measuring interleukin-8(IL-8) and C-reactiveprotein(CRP) in induced sputum by ELISA for 25 acute exacerbation patients with COPD,25 stable patients with COPD and 25 healthy control subjects.Total white cell numbers and the percent of neutrophils and eosnophils were counted by Wright-Giemsa Stain and under microscope.Results The FEV1.0%pred was lower in acute exacerbation patients with COPD(39.72±8.3)% than in stable patients with COPD(59.36±3.37) %(P0.01) and healthy control subjects(86.21±6.52)%(P0.01).In induced sputum the level of IL-8 and CRP was higher in acute exacerbation patients with COPD [(25.39±6.77)ng·L-1 and(8.39±3.82)g·L-1] than in stable patients with COPD[(6.14±2.26)ng·L-1、(4.67±2.13)g·L-1](P0.01) and healthy control subjects [(2.44±0.63)ng·L-1、(0.09±0.21)g·L-1](P0.01).In induced sputum the total white cell numbers and the percent of neutrophils was higher in acute exacerbation patients with COPD [(8.7±6.9)×109 L-1、(64.3±9.2)%) ] than in stable patients with COPD[(5.2±4.8)×109 L-1、(52.9±9.9)%](P0.01) and healthy control subjects [(2.3±1.4)×109 L-1、(28.6±7.9)%](P0.01).Conclusions The neutrophilic infiltrate,IL-8 and CRP rise within air passage are included in COPD,and it is aggravated along with inflammation exacerbation.So it is key to control infectious inflammation for preventing and relieving COPD.