ScholarLens Back to search
2005•Chinese Journal of Cardiovascular MedicineRequires access

Effect and Mechanism of oxidized LDL on U937 cell apoptosis

Lingyu Wang

Open publisher page 0 citations

Abstract

Objective To investigate the mechanism of ox-LDL inducing U937 cell apoptosis. Methods U937 cell were incubated and treated with different concentrations(0 to 300μg/ml) of ox-LDL for 24 hours,and with 100μg/ml ox-LDL for different time (0～72h). The degree of U937 cell apoptosis was determined by flow cytometry. The expression of caspase3,8,9 was detected by immune histochemsitry. Results Incubation U937 cell with ox-LDL, the apoptosis of U937 cell and the expression of caspase3, 8, 9 were increased. Conclusions ox-LDL can induce U937 cell apoptosis, and the ox-LDL-induced apoptosis involved both death receptor and mitochodria pathway.

About this research paper

What this paper is about

Objective To investigate the mechanism of ox-LDL inducing U937 cell apoptosis. Methods U937 cell were incubated and treated with different concentrations(0 to 300μg/ml) of ox-LDL for 24 hours,and with 100μg/ml ox-LDL for different time (0～72h). The degree of U937 cell apoptosis was determined by flow cytometry. The expression of caspase3,8,9 was detected by immune histochemsitry. Results Incubation U937 cell with ox-LDL, the apoptosis of U937 cell and the expression of caspase3, 8, 9 were increased. Conclusions ox-LDL can induce U937 cell apoptosis, and the ox-LDL-induced apoptosis involved both death receptor and mitochodria pathway.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To investigate the mechanism of ox-LDL inducing U937 cell apoptosis. Methods U937 cell were incubated and treated with different concentrations(0 to 300μg/ml) of ox-LDL for 24 hours,and with 100μg/ml ox-LDL for different time (0～72h). The degree of U937 cell apoptosis was determined by flow cytometry. The expression of caspase3,8,9 was detected by immune histochemsitry. Results Incubation U937 cell with ox-LDL, the apoptosis of U937 cell and the expression of caspase3, 8, 9 were increased. Conclusions ox-LDL can induce U937 cell apoptosis, and the ox-LDL-induced apoptosis involved both death receptor and mitochodria pathway.

Key concepts: U937 cell, Apoptosis, Flow cytometry, Cell, Medicine, Receptor, Programmed cell death, Incubation

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Effect and Mechanism of oxidized LDL on U937 cell apoptosis — Research Paper | ScholarLens