2007•Prevention and Treatment of Cardio-Cerebral-Vascular DiseaseRequires access
The Effect of LOX-1 on U937 Cell Apoptosis Induced by Oxidized LDL and the Protective Effect of Captopril
Zheng Yan
Open publisher page 0 citations
Abstract
Objective To investigate the effect of LOX-1 on U937 cell apoptosis induced by oxidized LDL and the protective effect of Captopril. Methods U937 cells were preincubated with different LOX-1 inhibitors and captopril for 1 hour,then incubated with ox-LDL 100ug/ml for 24 hours.The change of apoptosis and proteolytic enzymes was observed.The degree of U937 cell apoptosis was determined by flow cytometry.The expression of caspase 3,8 and 9 was detected by immunohistochemistry and Western Blot.Results Incubation U937 cell with ox-LDL can induce U937 cell apoptosis and significant upregulate the expression of caspase 3,8 and 9.Preincubation U937 cell with LOX-1 inhibitors PIA,carrageenan and captopril for 1 hour could significantly suppresse U937 cell apoptosis induced by ox-LDL and restraine the increase of caspase 3,8 and 9(P0.05).Conclusions LOX-1 played a key role on U937 cell apoptosis induced by ox-LDL.Ox-LDL/LOX-1 interaction on U937 cell can induce apoptosis via death receptor and mitochondria pathway.Captopril can protect U937 cell from ox-LDL induced apoptosis.