Technology reseach of enzymatic hydrolysis for the muscle of carp

The optimum conditions of trypsin,subtilisin,and pepsin enzymatic hydrolysis for carp was studied,and the effect of enzymatic hydrolysis was compared.Orthogonal experiment and comparative research were used to study enzymatic hydrolysis conditions of carp,and analyze the content of free Amino acids before and after enzymatic hydrolysis for carp.The optimum conditions for the trypsin enzymatic hydrolysis were enzyme concentration 24 g/kg,enzymatic temperature 50 ℃,and pH 7.0.Under this condition the content of amino nitrogen was 59.2 mg/L after 3 hours of enzymatic hydrolysis.The optimum conditions for the Subtilisin were enzyme concentration 0.16 g/kg,enzymatic temperature 45 ℃,pH8.0,and the content of amino nitrogen was 44.6 mg/L after 4 hours.The optimum conditions for the Pepsin Enzymatic hydrolysis were enzyme concentration 10 g/kg,enzymatic temperature 60 ℃,pH 7.0,and the content of amino nitrogen was 102.6 mg/L after 4 hours of enzymatic hydrolysis.After subtilisin enzymatic hydrolysis for carp,the content of free Amino acids increased from 185.91 mg/L to 779.91 mg/L,increasing by 76.16%.Through comparing the three protease enzymatic hydrolysis for carp,subtilisin was the better protease for carp.