Study on CD4~+CD25~+CD127~(low) regulatory T cells and related cytokines in patients with idiopathic thrombocytopenic purpura

Objective:To investigate the amount of CD4+CD25+CD127low regulatory T cells and related cytokines in peripheral blood of the patients with idiopathic thrombocytopenic purpura(ITP),and discuss the role they play in the pathogenesis of ITP.Methods:The amount of CD4+CD25+CD127low regulatory T cells in the peripheral blood of 31 ITP patients and 25 healthy donors were evaluated by flow cytometry,ELISA was used to test the level of IL-10 and TGF-β1 in blood serum,and then we analyzed the correlation between levels of CD4+CD25+CD127low regulatory T cells and IL-10,TGF-β1.Results:ITP patients had a lower proportion of CD4+CD25+CD127low regulatory T cells than the healthy donors(P0.05).The level of IL-10 and TGF-β1 in ITP patients was also lower than that in healthy donors(P0.05).There was positive correlation between levels of CD4+CD25+CD127low regulatory T cells and IL-10(P0.05).There was no positive correlation between levels of CD4+CD25+CD127low regulatory T cells and TGF-β1(P0.05).Conclusion:Decrease of CD4+CD25+CD127low regulatory T cells and related cytokines in ITP patients may be connected with their cellular immunity disturbance.