Expression of CD4~+ CD25~+ regulatory T cells and TGF-β1 in patients with idiopathic thrombocytopenic purpura

AIM: To investigate the expression rate of CD4+CD25+ regulatory T cells and TGF-β1 in peripheral blood of the patients with idiopathic thrombocytopenic purpura(ITP),and the role they play in the pathogenesis of ITP.METHODS: The population of CD4+CD25+ regulatory T cells in the peripheral blood of 31 ITP patients and 25 healthy donors was evaluated by flow cytometry,ELISA was used to test the level of TGF-β1 in blood serum,and analysed the correlation between levels of CD4+CD25+ regulatory T cells and TGF-β1.RESULTS: ITP patients had a lower proportion of CD4+CD25+ regulatory T cells than the healthy donors(P0.05),The level of TGF-β1 in ITP patients was also lower than healthy donors(P0.05),There was not positive correlation between levels of CD4+CD25+ regulatory T cells and TGF-β1(P0.05).CONCLUSION: The results indicate that the decreasing of CD4+CD25+ regulatory T cells in ITP patients may be connected with cellular immunity disturbance of idiopathic thrombocytopenic purpura.Further Research need to be perforrmed in metabolic changes on CD4+CD25+ regulatory T cells and TGF-β1 in patients with idiopathic thrombocytopenic purpura.