Effects of IGF-II on MC3T3-E1 Cells Proliferation and Nitric Oxide Production

ObjectiveTo study the effects of IGF-II on cell proliferation and nitric oxide content in MC3T3-E1 osteoblast.MethodsMouse calvaria-derived osteoblastic clone MC3T3-E1 cell line were incubated with different concentrations of IGF-II in α-MEM culture medium. Cell proliferation was determined by MTT and the nitric oxide concentration in the culture medium was measured by enzyme assay. ResultsOver a concentration range of 1～100 ng/mL, IGF-II promoted MC3T3-E1 cells proliferation in a dose-dependent manner, and it also significantly decreased the level of nitric oxide in the 100 ng/mL group at the third day (P0.01); the OD value of the control group at the sixth day was evidently lower than that of at the fifth day (P(0.01)),while the OD value of the 100 ng/mL group at the sixth day was obviously higher than that of at the fifth day (P0.01). ConclusionIGF-II can promote cell proliferation, inhibit NO production, and can significantly prevent cell death of MC3T3-E1.