Effects of Ginkgo biloba extract on liver HDAC2 expression in acute liver failure

Objective To study the protection effect of ginkgo biloba extract(GBE) on TAA-induced acute liver failure(ALF).Methods 46 rats were divided into five groups: control,model and GBE groups.The model mice of ALF were set up by giving intraperitoneal injection of TAA(350 mg/kg).The serum ALT and AST were determined,HDAC2 was analyzed with RT-PCR.The protein expression of HDAC2 was analyzed with Western blot.Results The serum ALT and AST in mice of model group were increased significantly compared with those of control group.The serum ALT and AST in mice of GBE group were decreased significantly compared with model group.Compared with model group,the gene and protein expressions of HDAC2 were significantly up-regulated in liver of GBE group.Conclusions GBE could protect hepatocytes from apoptosis and necrosis in acute liver injury by TAA in rat and the mechanisms is associated with HDAC2.