Relationship of liver fibrosis and inflammation between connective tissue growth factor in liver tissue of experimental rat

[Objective] To reveal the pathogenesy of connective tissue growth factor (CTGF) in liver fibrosis by observing the relationship of liver fibrosis and inflammation and CTGF in liver tissue of experimental rat. [Methods] The immune liver fibrosis model in rats were established by injected human albumin into vena caudalis.The expression of CTGF in liver tissues were detected by immunohistochemistry and RT-PCR. [Results] ①The positive cells of immunohistochemistry of CTGF distribute at portal area, fabric interval, juncture with liver parenchyma and perisinusoidal cells. It is negative in parenchymal hepatic cells and normal group. ②With the development of fibrosis, the expressions of CTGF and it′s mRNA in liver tissues increase gradually from S0 to S4. Compared to S0, S1, S2, S3 and S4 are all significantly higher (P 0.05). And there are significant differences between each of them (P 0.05). ③With the development of inflammation and cellular necrosis, the expressions of CTGF and it′s mRNA in liver tissues increase gradually from G0 to G4. Compared to G0, G1, G2, G3 and G4 are all significantly higher (P 0.05). The difference between G1 and G2 are notable (P 0.05). But the differences between G2 and G3, G3 and G4 are unnotable (P 0.05). [Conclusions] CTGF pays an important role of the devolpment of liver fibrosis.