Effects of hepatis B vines on the growth and cell subsets analysis of cytokine-induced killer cells(CIK) in vitro incubation from tumor

Objective To explore the difference of the immune cell subsets during the process of cytokine-induced kill cells(CIK) incubation from tumor patients with and without hepatitis B virus.Methods The peripheral blood was extracted from 50 tumor patients from Oct 2012 to Jun 2013 who were divided into two groups by with or without hepatitis B virus.The proliferate velocity and activity of CIK cells were detected by count on 1,5,7,9,11,13 and 15 days of the CIK culture.The expressions of CD3~+,CD4~+,CD8~+,CD3~+ CD8~+,CD3~+ CD4~+,CD3~+ CD56~+ T cells were analyzed by flow cytometry on 5,7,10,13 and 15 days of the culture.Results The proliferative multiple at 15 days in HBV positive group was higher than that of negative group(280 vs.180),although there was no significant difference between the two groups at the same culturing time points.The frequencies of CD3~+T cells,CD8~+T cells,CD3~+CD8~+T cells and CD3~+ CD56~+T cells increased by the time,whilethe CD4~+T cells and CD3~+CD4~+T cells decreased by the time,the positive group was more than the negative.Among them,the difference of CD8~+T cells and CD3~+CD4~+T cells in two groups was significant(P0.05).Conclusion The proliferate multiple capacity of CIK cells from patients with HBV positive group was better than that of negative group,and the immune cell subsets states of HBV positive group was better than that of negative group..