ScholarLens Back to search
2005•Journal of Preventive Medicine of the Chinese People's Liberation ArmyRequires access

EXPRESSION AND PURIFICATION OF RECOMBINANT HUMAN ENDOSTA TIN AND ITS EFFECT ON ENDOTHELIAL CELL PROLIFERATION

Jie Yang

Open publisher page 0 citations

Abstract

Objective To investigate the effect of recombinant human endostatin expressed in E.coli on the proliferation of ECV304 cells.Methods pTRX-endo plasmid was expressed in E.coli BL21(DE3).Endostatin was expressed in E.coli by IPTG inducement, ECV304 cell were added to it for culture after purification, and its prolification effect was analyzed by MTT.Results SDS-PAGE analysis showed a specific protein strip,the purity of recombinant human endostatin was over 95% after purification. Endostatin inhibited obviously the proliferation of ECV304 cells. Conclusion Recombinant human endostatin may antagonize the proliferation of endothelial cell.The experiment lays foundation for the research of antiangiogenesis and application of endostatin.

About this research paper

What this paper is about

Objective To investigate the effect of recombinant human endostatin expressed in E.coli on the proliferation of ECV304 cells.Methods pTRX-endo plasmid was expressed in E.coli BL21(DE3).Endostatin was expressed in E.coli by IPTG inducement, ECV304 cell were added to it for culture after purification, and its prolification effect was analyzed by MTT.Results SDS-PAGE analysis showed a specific protein strip,the purity of recombinant human endostatin was over 95% after purification. Endostatin inhibited obviously the proliferation of ECV304 cells. Conclusion Recombinant human endostatin may antagonize the proliferation of endothelial cell.The experiment lays foundation for the research of antiangiogenesis and application of endostatin.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To investigate the effect of recombinant human endostatin expressed in E.coli on the proliferation of ECV304 cells.Methods pTRX-endo plasmid was expressed in E.coli BL21(DE3).Endostatin was expressed in E.coli by IPTG inducement, ECV304 cell were added to it for culture after purification, and its prolification effect was analyzed by MTT.Results SDS-PAGE analysis showed a specific protein strip,the purity of recombinant human endostatin was over 95% after purification. Endostatin inhibited obviously the proliferation of ECV304 cells. Conclusion Recombinant human endostatin may antagonize the proliferation of endothelial cell.The experiment lays foundation for the research of antiangiogenesis and application of endostatin.

Key concepts: Endostatin, Recombinant DNA, Cell growth, Cell, Molecular biology, Chemistry, VEGF receptors, Biology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
EXPRESSION AND PURIFICATION OF RECOMBINANT HUMAN ENDOSTA TIN AND ITS EFFECT ON ENDOTHELIAL CELL PROLIFERATION — Research Paper | ScholarLens