Endostatin Inhibits Proliferation of Vascular Endothelial Cells Mediated by TNFαand IL-8 and Down-regulates Expression of Adhesive Molecules

Objectives: The current study was designed to investigate the inhibitive effect of recombinant human endostatin on the proliferation of human endothelial cells of vessels (ECV) mediated by TNFα and IL-8. The influence of endostatin on the expression of adhesive molecules on the surface of ECV was also studied. Methods: The effect of endostatin on the proliferation of ECV in vitro was examined using MTT assay. The expression of adhesive molecules on the surface of ECV was determined by flow cytometry. Results: The proliferation of ECV was inhibited significantly after ECV was incubated with 100 - 10 000 ng/ml of endostatin for 72h. The stimulating effect of TNFα and IL-8 on ECV was also suppressed markedly by endostatin. A dose-dependent inhibition was observed. At the level of 100 - 1000 ng/ml, endostatin also inhibited significantly the expression of CD62E and CD40 on the surface Of ECV. Conclusions: Endostatin not only exerts an inhibitive effect on the proliferation of endothelial cells, which may be helpful to the control of angiogenesis in solid tumors, but also suppresses the expression of adhesive molecules on the surface of endothelial cells. Thus, endostatin maybe play an important role in dealing with the metastasis of solid tumor.