Prokaryotic Expression and Optimization of Expression Conditions for Human Acetaldehyde Dehydrogenase 2

The human acetaldehyde dehydrogenase 2(ALDH2)gene was cloned into prokaryotic expression vector pGEX-4T-1with fusion protein tag GST to construct prokaryotic expression recombinant plasmid pGEX-4T-1-ALDH2.The recombinant plasmid was transformed into E.coli BL21(DE3).After being induced by IPTG,expression of the purpose protein was verified by SDS-PAGE analysis,and through optimizing the expression conditions(0.1mmol·L-1 IPTG,28 ℃,10h),the purpose protein present in supernatant achieved25%of that in pellet of the bacterial lysate because of the fusion protein tag GST.It showed that the fusion protein tag GST and low temperature could improve soluble expression of ALDH2in Escherichia coli.