Expression and Purification of Soluble OsVDAC3 in vitro

[Objective]To achieve activated OsVDAC3 protein in vitro.[Method]The recombinant prokaryotic expression vector pGEX-4T-1-osvdac3 with the GST-tag was constructed and transformed into E. coli. TheGst-tagged OsVDAC3 protein was induced with IPTG in E. coli strain BL21,and confirmed by SDS-PAGE and Western-Blot analysis. In addition,the GST-tagged recombinant OsVDAC3 protein was purified by Glutathione resin.[Result] The results showed that the recombinant prokaryotic expression vector pGEX-4T-1-osvdac3 was constructed and transformed into E. coli successfully. The results of SDS-PAGE and Western Blot showed a band in the 56 kD and indicated the GST-tag OSVDAC3 protein was partly existed in the soluble composition.[Conclusion]The GST-tag OsVDAC3 protein was isolated and purified by using Glutathione Resin. The research laid a foundation for the further study of the function of VDAC in the rice.