Intracellular Expression of Lateolabrox japonicus Growth Hormone in Methyltrophic Yeast, Pichia pastoris.

The methylotrophic yeast, Pichia pastoris heterologous gene expression system was utilized to produce attractive levels of a variety of intracellular and extracellular proteins of interest. Lateolabrox japonicus growth hormone gene was cloned into the yeast integrative vector pHILD2, which was then transformed into his4 mutant yeast GS115. PCR fast detection methods was performed to screen the positive transformants and dotblotting was used to screen the multiplecopy transformants. Via inducing AOX1 promoter by methanol, growth hormone could be expressed intracellularly. SDSPAGE and Western blot were applied to confirm the product.