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2011•The Chinese Journal of Modern Applied PharmacyRequires access

Quantitative Determination of Quercetin in the Lipid-regulation and anti-Oxidation Effective Fraction of Jiangzhining by HPLC

Bin Liu

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Abstract

OBJECTIVE To establish a method for the quantitative determination of quercetin in the lipid-regulation and anti-oxidation effective fraction of Jiangzhining.METHODS The determination was carried out with SunFire C18 column(150 mm × 4.6 mm,5 μm),using acetonitrile-0.1% phosphoric acid water solution(30︰70) as mobile phase at a flow rate of 1.0 mL·min-1 and detected at the wavelength 370 nm,column temperature 30℃.RESULTS Quercetin had a good linear relationship in the range of 0.052 2-0.313 2 μg with the linear regression equation was Y=3 231 057.74X-4 753.64(r=0.999 5),the average recovery was 99.36% with RSD=1.73%(n=6).CONCLUSION The determination results of three batches of samples showed that the method was easy and accurate which could be used to determine the content of quercetin in the effective fraction of Jiangzhining.

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OBJECTIVE To establish a method for the quantitative determination of quercetin in the lipid-regulation and anti-oxidation effective fraction of Jiangzhining.METHODS The determination was carried out with SunFire C18 column(150 mm × 4.6 mm,5 μm),using acetonitrile-0.1% phosphoric acid water solution(30︰70) as mobile phase at a flow rate of 1.0 mL·min-1 and detected at the wavelength 370 nm,column temperature 30℃.RESULTS Quercetin had a good linear relationship in the range of 0.052 2-0.313 2 μg with the linear regression equation was Y=3 231 057.74X-4 753.64(r=0.999 5),the average recovery was 99.36% with RSD=1.73%(n=6).CONCLUSION The determination results of three batches of samples showed that the method was easy and accurate which could be used to determine the content of quercetin in the effective fraction of Jiangzhining.

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Available abstract

OBJECTIVE To establish a method for the quantitative determination of quercetin in the lipid-regulation and anti-oxidation effective fraction of Jiangzhining.METHODS The determination was carried out with SunFire C18 column(150 mm × 4.6 mm,5 μm),using acetonitrile-0.1% phosphoric acid water solution(30︰70) as mobile phase at a flow rate of 1.0 mL·min-1 and detected at the wavelength 370 nm,column temperature 30℃.RESULTS Quercetin had a good linear relationship in the range of 0.052 2-0.313 2 μg with the linear regression equation was Y=3 231 057.74X-4 753.64(r=0.999 5),the average recovery was 99.36% with RSD=1.73%(n=6).CONCLUSION The determination results of three batches of samples showed that the method was easy and accurate which could be used to determine the content of quercetin in the effective fraction of Jiangzhining.

Key concepts: Quercetin, Chemistry, Chromatography, Phosphoric acid, Fraction (chemistry), Linear range, Linear relationship, Acetonitrile

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