Molecular cloning and characterization of thymosin β_(11) cDNA in grass carp

The thymosinβ_(11) EST sequence was picked out from the cDNA library of Ctenopharyngodon idellus to design the RACE primers.The full length cDNA sequence of thymosinβ_(11)(GenBank accession number:HM120850) from C.idellus intestine was cloned by rapid amplification of cDNA ends(RACE) method.The full langth of this gene's cDNA is 320 bp.It has a 47 bp 5'-untranslated region(UTR) and a 141 bp 3'-UTR.The open reading frame(ORF) of this gene's cDNA is 132 bp which putatively encodes 43 amino acids with the isoelectric point of 5.17 and molecular weight of 4892 Da.Multiple alignments showed that the deduced amino acid sequence ofβ-thymosins from grass crap shares a high degree of identity withβ-thymosins sequences from other species,and the highest homology specie is Danio rerio,(93.0%).Secondary structure prediction analysis indicated that secondary structure of C. idellu's thymosinβ_(11) is highly similar with otherβ-thymosins.