Analysis of polymorphism at position +8006 in exon 2 of interleukin-1 receptor antagonist in patients with acute myocardial infarction

Objective To assess the correlation between polymorphism of interleukin-1 receptor antagonist gene (IL-1RN)and risk of acute myocardial infarction (AMI), and its modulation on the serum interleukin-1β(IL-1β), interleukin-1 receptor antagonist (IL-1Ra) and C-reactive protein(CRP)level in patients with AMI. Methods The polymorphism of IL-1RN was detected by a combination of polymerase chain reaction-restriction fragment length polymorphism methods in 178 AMI patients and 190 healthy controls, then the serum IL-1β, IL-1Ra and CRP levels in 32 cases random AMI patients were inspected by enzyme-linked immunosorbent assay. Results There is a significant difference of distribution of IL-1RN polymorphism in between the AMI and control groups ( P 0.01). No IL-1RN*C carriers showed an increased risk with an odds ratio of 3.01 for AMI (OR=3.01, 95%CI=1.51-6.03) compared with healthy controls. The serum IL-1Ra level of IL-1RN*C carriers was significantly higher than that of non-IL-1RN*C carriers in patients with AMI. Conclusion The IL-1RN polymorphism is associated with AMI and may affect the modulation on serum IL-1Ra level, but not IL-1β and CRP level.